The evaluation of gametic selection in barley anther culture in vitro by endosperm protein markers

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Belinskaya E.V., Parkhomenko R.G., Kopitina L.P.

Yurjev Plant Production Institute, 142 Moscowsky Av., Kharkov, 310060, Ukraine

Our previous study on the androgenetic ability of two spring barley varieties Kharkovsky 74 (responsive) and Kharkovsky 67 (non-responsive), their reciprocal F1 crosses and 13 androgenetic doubled haploid lines derived from these crosses has shown the intensive in vitro selection for increased anther culture response. Some DH- lines possessed much higher culturability than parental varieties and crosses. It may be suggested that anther culture in vitro per se promotes the preferential development of microspores with certain chromosome composition favoring androgenesis in vitro and thus reduces the ability of haploid production from a random sample of gametes.

The aim of this research was the evaluation of gametic selection in vitro by PAGE electrophoretic analysis of storage proteins of genotypes mentioned above. It is important to note that parental varieties are distinguished in hordein spectrum.

The results of our investigation have revealed 4 types of electrophoretic spectrum among 13 DH-Iines instead of two expected. In particular, it has appeared that the hordein composition of five lines was similar to that one of Kharkovsky 74. The hordein spectrum of three lines was similar to that one of Kharkovsky 67. Doubled haploid lines 8(l)/95 and 8(2)/95 had the allelic variant of HrdB differed from that one of both parents. The rest three lines had a recombinant phenotype. The analysis of data has indicated that the inheritance of hordein composition had no significant effect on the androgenetic ability. As the loci encoded the hordein spectrum are located on the short arm of the fifth chromosome we can make a conclusion that this chromosome does not play an important role in the determination of androgenetic ability in spring barley. Isozyme and DNA markers must be involve into the further investigations on the gametic selection in anther culture in vitro of spring barley.